The 3th 1 you need is a specific program for trojans,1 of the best is TrojanHunter.My 1st security line is covered by Avast Pro,Outpost Pro and TrojanHunter;the 2nd defense line is AdAware's Adwatch,Microsoft AntiSpyware and Spybot's TeaTimer;all of them monitoring in realtime....
Periodicly I let them all run their scanning engine....
Antispyware programs can recognise a few trojans,as do most virusscanners,but not enough to be a secure antitrojan solution...
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